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. 2022 Jul 22;29(12):1918–1929. doi: 10.1038/s41417-022-00507-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Cell viability of JAM-A negative (PN014) and JAM-A positive (PN021) human pancreatic fibroblasts infected with R124 and jin-3 at different MOIs. B Western blot for σ3 protein in JAM-A negative and JAM-A positive human fibroblasts infected with R124 and jin-3 for indicated time points. M = mock, R = R124, J = jin-3 C S4 copy number as determined by RT-qPCR in JAM-A negative and JAM-A positive human fibroblasts infected with R124 and jin-3 for indicated time points. D Immunofluorescent staining of KPC3 subcutaneous tumors for vimentin and σ3 protein after reovirus infection (REO1, REO2) or mock control (CTR1, CTR2). E Flow cytometry of murine CAF cultures derived from KPC3 tumors for expression of JAM-A. F Viability of JAM-A negative (KPC3-CAF1) and JAM-A positive (KPC3-CAF3) murine fibroblast cultures 5 days post-infection with R124 or jin-3. Two-way ANOVA for all different virus conditions, *** = P ≤ 0.0001. G Western blot for σ3 protein in JAM-A negative and JAM-A positive murine fibroblasts infected with R124 and jin-3 for indicated time points. M = mock, R = R124, J = jin-3 H S4 copy number as determined by RT-qPCR in JAM-A negative and JAM-A positive murine fibroblasts infected with R124 and jin-3 for indicated time points.