Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jul 4;40(12):1823–1833. doi: 10.1038/s41587-022-01379-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 3 — a,d Quantification of single cell events using individual markers and marker combinations (including standard cell types). Total cell counts to define percentage of positivity were between 3,000 to 5,000 cells per assay area and were matched ± 300 total cells for paired samples (experimental vs control region). Minimum population proportion within 5% margin of error and 95% confidence level was set to 0.75% (represents 12 cells) to discriminate noise from specific signal. Bars are mean ± s.e.; n = 3 reservoirs from two to three tumors. MMTV-PyMT mice with late stage (d93-d100) spontaneously growing tumors implanted with IMD for three days were used. Significance was calculated by paired sample one tailed t-test. b, Gray scale single channel images of depicted markers at the PEG-delivery reservoir. Arrow indicates the source and direction of PEG release. Scale bar; 100 µm. c, Presentation of main response cell types (biomarker combination displayed) in XY space. Coordinate [0,0] identifies the drug source. The direction of the drug release is upward.