Table 1.

Recipe for preparing transfection mixture for transfection of one, 100 mm petri-dish

Reagent	Final concentration	Amount
pLentiGFP/Anti-CSP IB construct	10 μg/mL	10 μg
pCMVR8.74	9.8 μg/mL	9.8 μg
Spike construct	6 μg/mL	6 μg
Tat plasmid	6 μg/mL	6 μg
Rev plasmid	6 μg/mL	6 μg
polyethyleneimine (PEI)	1 μg/mL	114 μL
Serum Free DMEM	ad	1 mL