REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-c-Myc tag antibody (1:2000)	Santa Cruz Biotech (SCBT)	Cat#9E10
Streptavidin APC (1:200)	BioLegend	Cat#405207
Mouse anti-6×(HIS) tag (1:5000 for WB, 1:100 in confocal)	Invitrogen	Cat#4A12E6
Alkaline phosphatase-conjugated goat anti-mouse IgG (1:10000 for WB)	Sigma	Cat#A3562
Anti-Ubiquitin antibody (1:2500)	SCBT	Cat#Sc8017
Anti-GAPDH antibody (1:1500)	Invitrogen	TG267131
Golgin (rabbit) (1:100)	Sigma	PA5-52841
Calnexin (rabbit) (1:100)	Cell Signal Technology (CST), Beverly, Massachusetts, USA	52533S
Goat anti-mouse Alexa Fluor 647 (1:100)	Thermo Fisher Scientific	A21245
Goat anti-Rabbit Alexa fluor 568 (1:100)	Thermo Fisher Scientific	A11036
Hoechst stain (10 μg/mL)	Thermo Fisher Scientific	62249
Streptavidin HRP (1:500)	Life Technology	SNN1004
Anti-Flag mouse antibody (1:2500)	Sigma	F1804-50UG
Anti-CSP sdAb (10 μg/mL)	In-house generated	Ref.1
Chemicals, peptides, and recombinant proteins
Tunicamycin	Sigma	T7765-5MG
PVDF membrane	Bio-Rad	Cat#1620177
Trypsin	HiMedia	59427C-500mL
Bovine serum albumin	HiMedia	GRM105-100G
Ni- NTA beads	G Biosciences	Cat#786940
TMB substrate for ELISA	Becton, Dickinson and Company (BD)	Cat#555214
FemtoLUCENT plus AP kit	G-Biosciences	Cat#786-10AP
PEI branched	Sigma	408727-100mL
BamHI	New England Biolab (NEB)	R3136S
XbaI	NEB	R0145S
NcoI	NEB	R3193S
NotI	NEB	R3189S
MG132	Sigma	474787
Ni-NTA probes	ForteBio	18-5101
Clarity Western ECL substrate	Bio-Rad	Cat#170-5060
pNPP substrate	Sigma	71768-25G
DMEM	Gibco	10566-016-500mL
RPMI	Gibco	A10491-01 500mL
Fetal bovine serum	Gibco	10270106- 500 mL
NSPRRARSVAS (cleavage site peptide)	S-Biochem	Used in this study
Recombinant DNA
WT SARS-CoV-2 S plasmid	Dr. Jason McLellan (University of Texas)4	https://doi.org/10.1126/science.abb2507
pCMVR8.74	Dr. Indranil Banerjee (IISER Mohali)	#22036
pMD2.G	Dr. Indranil Banerjee	#12259
pCMV14-3×-FLAG-SARS-CoV-2 S	Addgene	#145780
pLenti-GFP	Addgene	#17448
Tat 1b and Rev 1b	SARS-Related Coronavirus 2, Wuhan-Hu-1 spike-pseudotyped lentiviral kit	NR-52948
pYBNT (modified pet 22b) Anti-CSP-IB clone	Novagen In-house generated	pET22b+ Used in this study (Described earlier)5
Experimental models: Cell lines
HEK293T cells	ATCC	CRL-3216™
Vero E6 cells	Dr. Rajesh Ringe lab (IMTECH, CHANDIGARH)	NA
Vero cells	ATCC	CCL-81
Experimental models: Organisms/strains
DH5 alpha-competent cells	Ref.5	Ref.5
Origami competent cells	Ref.5	Ref.5
Stbl3 competent cells	Thermo Fisher Scientific	Cat#C7381201
Software and algorithms
FlowJo software	BD Biosciences	https://www.flowjo.com/solutions/flowjo/downloads
Image J	National Institute of Health (NIH), MD	https://imagej.nih.gov/ij/download.html
Immune Epitope Data Base	National Institute of Allergy and Infectious Diseases (NIAID), MD	http://tools.iedb.org/main/bcell/
BLItz software	ForteBio	http://www.blitzmenow.com
GraphPad Prism 9	GraphPad	https://www.graphpad.com/scientific-software/prism/
Other
Flow cytometer	Accuri-C6	BD
Fluorescence microscope	Nickon	Nikon eclipse Ti
Confocal microscope	Leica	SP8 upright
ELISA plate reader	Biotech	Eon
CO2 incubator	Eppendorf	Galaxy 170 R
Microbial incubator	Labquick	Talboys
Biosafety cabinet	Labconco	Logic+
Centrifuge	Eppendorf	5810R
Rotospin	Tarsons	Tarson
100-mm petri dishes	CLS430167-500EA	Merck
96 well-flat bottom plates	3799	Corning
Glass slides	Frosted Micro-slides	Blue star
Coverslips	Microscopic cover glass	Blue star
X-ray film	Edusoft Healthcare	E-Ray
FP VHH FR1 pLenti: 5′GCATT CTAGAGGCACCCCGGGATG GCCGATGTTCAACTGCAGGAG3′	GCC Biotech India	Ref.1
FR4 C-Myc pLenti: 5′GCTAGG ATCCTGCAGATCCTCTTCAGAG ATGAGTTTCTGCTCTGTGGAGA CGGTGACCTG3′	GCC Biotech India	Ref.1
MKS-9 (VHH FP): 5′GTTGTGT GGAATTGTGAGCG3′	GCC Biotech India	Ref.5
MKS-22 (VHH RP): 5′GAAATGCGG CCGCTGTGGAGACGGTGACCTG3′	GCC Biotech India	Ref.5
T7-FP: 5′TAATACGACT CACTATAGGGGAATTGTG-3′	GCC Biotech India	Ref.5
T7-RP: 5′GCTAGTTATTGCT CAGCGGTGGCAGCAGC3′	GCC Biotech India	Ref.5