| Buffer III (neutralization buffer) | Final conc. | Amount |
|---|---|---|
| Potassium acetate | 5 M | 41.01 g |
| Glacial Acetic Acid | 11.5% | 11.5 mL |
| Milli-Q water | Ad | 100 mL |
Autoclaved for 15 min at 121°C and keep at 4°C.
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