Fig. 4.

CTSLP8 bound to PKM2 directly and positively correlated with c-Myc expression. A RNA pull-down combined with mass spectrometry showed the proteins binding directly to CTSLP8. B RNA pull-down combined with WB showed that CTSLP8 binds to PKM2 directly. C RIP assays verified that PKM2 binds to CTSLP8. D FRET confirmed that CTSLP8 interacted with PKM2. E–F The changes in levels of CTSLP8 neither affected the expression of PKM2 in mRNA level nor the protein level. G–H RT-PCR and western blot showed the expression of c-Myc in OC cell lines with different CTSLP8 expressions. I RT-PCR and western blot showed that upregulation of PKM2 expression promoted the expression of c-Myc in P8-NC/P8-OE cell lines