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. 2022 Dec 1;13:1074153. doi: 10.3389/fmicb.2022.1074153

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Copyright © 2022 Wang, Lang, Zhang, Zhai, Zheng, Chen, Liu and Zheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The Core-20 community stably colonized honeybees for four passages. (A) Experimental design for passaging transmission. (B) Relative abundance of strains in the Core-20 during four passages. All strains except G. sp. W8131 were detected individually in bee gut samples among passages. (C) Box plots of Shannon’s alpha diversity index at each passage. Results showed a slight decrease in alpha diversity. (D) Principal coordinates analysis (PCoA) plot of Bray–Curtis dissimilarity among samples. We found that the last three passages showed community similarity except for P1.