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OB upregulated the anabolism in IL-1β–induced chondrocytes. Chondrocytes were treated with IL-1β (5 ng/ml) or/and OB (160 μM) for 24 h. (A) Western blot analysis and (B) quantitative data for the key anabolic-related proteins (Aggrecan, Collagen II). (C, E) Immunofluorescence staining and (D, F) quantitative data of the expression levels of Aggrecan and Collagen II. Data were presented as means ± SD (n = 3). ns, no significance; *p < 0.05; **p < 0.01; ***p < 0.001.
