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. 2022 Dec 1;13:1060721. doi: 10.3389/fendo.2022.1060721

Figure 7.

Figure 7

3-MA reversed the anti-cartilage degradation and the anti-inflammatory effects of OB in IL-1β–induced chondrocytes. Chondrocytes were administered with IL-1β (5 ng/ml) or/and OB (160 μM) or/and 3-MA (5 mM)for 24 h. (A) Western blot analysis and (B) quantitative data for autophagy-related proteins (Atg3, Atg7, Beclin-1, P62, and LC3 I/II) in chondrocytes. (C) Western blot analysis and (D) quantitative data for anabolic and catabolic-related proteins (Aggrecan, Collagen II, MMP3, and MMP13). (E) Western blot analysis and (F) quantitative data for inflammatory proteins (iNOS, COX-2, TNF-α, and IL-6). Data were presented as means ± SD (n = 3). ns, no significance; *p < 0.05; **p < 0.01; ***p < 0.001.