Table 2.
Overview of markers used for the gene expression analysis to verify differentiation within the hematopoietic lineage or mesenchymal lineage and the top (up- and down-regulated) differentially expressed genes detected in the next generation RNA sequencing analysis of osteoprogenitor cells from responders and non-responders to supraphysiological loading.
| Gene name | Gene symbol | Assay ID |
|---|---|---|
| Hematopoietic Progenitor Cell Antigen CD34 | CD34 | Hs02576480_m1 |
| KIT Proto-Oncogene, Receptor Tyrosine Kinase | c-kit/CD117 | Hs00174029_m1 |
| Fms-Related Tyrosine Kinase 3 | FLT3/FLK2 | Hs00174690_m1 |
| Monocyte Chemoattractant Protein 1 | CCL2 | Hs00234140_m1 |
| Scavenger Receptor Cysteine-Rich Type 1 Protein M130 | CD163 | Hs00174705_m1 |
| Monocyte Differentiation Antigen CD14 | CD14 | Hs02621496_s1 |
| Nuclear Factor-κB Subunit 2 | NFKb2 | Hs00174517_m1 |
| Nuclear Factor of Activated T Cells 1 | NFATc1 | Hs00542675_m1 |
| Osteoclast-Associated, Immunoglobulin-Like Receptor | OSCAR | Hs01100185_m1 |
| Dendritic Cells (DC)-Specific Transmembrane Protein | DC-STAMP | Hs00229255_m1 |
| Hematopoietic Cell E- and L-Selectin Ligand | CD44 | Hs01075864_m1 |
| 5'-Nucleotidase Ecto | CD73 | Hs00159686_m1 |
| Thy-1 Membrane Glycoprotein | CD90 | Hs00174816_m1 |
| Homeobox Protein Hox-8 | MSX2 | Hs00741177_m1 |
| Runt-Related Transcription Factor 2 | RUNX2 | Hs00231692_m1 |
| Alkaline Phosphatase Liver/Bone/Kidney Isozyme | ALPL | Hs01029144_m1 |
| Collagen Type I Alpha 1 Chain | Col1A1 | Hs00164004_m1 |
| Aquaporin 1 | AQP1 | Hs01028916_m1 |
| Heart and neural crest derivatives expressed 2 | HAND2 | Hs00232769_m1 |
| Transforming acidic coiled-coil containing protein 3 | TACC3 | Hs01099874_m1 |
| Armadillo repeat gene deleted in velocardiofacial syndrome | ARVCF | Hs01554141_m1 |
| Transforming growth factor β2 | TGFB2 | Hs00234244_m1 |
| EGF-like repeats and discoidin domains 3 | EDIL3 | Hs00964112_m1 |
| FosB proto-oncogene, AP-1 transcription factor subunit | FOSB | Hs00171851_m1 |
| X inactive-specific transcript (non-protein coding) | XIST | Hs01079824_m1 |
| Hyaluronan and proteoglycan link protein 1 | HAPLN1 | Hs01091999_m1 |
| Suppressor of cytokine signaling 3 | SOCS3 | Hs02330328_s1 |
| Complement component 7 | C7 | Hs00940408_m1 |
| 18S Ribosomal RNA | 18S rRNA | Hs03003631_g1 |
| Beta-2-Microglobulin | B2M | Hs00187842_m1 |
| Hypoxanthine Phosphoribosyltransferase 1 | HRPT1 | Hs02800695_m1 |
| Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Zeta | YWHAZ | Hs01122445_g1 |