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. 2022 Jun 18;29(12):2445–2458. doi: 10.1038/s41418-022-01030-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Cells expressing the indicated p63 isoforms or as a control TAp73α, were fixed with formaldehyde and incubated with HA-tagged DARPin A5, followed by the goat anti-HA antibody (a190138a - Bethyl) and the secondary antibody Alexa Fluor 568 anti-goat (A11057—Life Technologies). The same cells were also stained with the mouse anti-myc antibody 4A6 (Millipore) and the secondary antibody Alexa Fluor 647 anti-mouse (A31571—Life Technologies) as all p63 isoforms and TAp73α are labeled with an N-terminal myc-tag. All SAM domain-containing p63 isoforms show strong staining while TAp63γ which lacks a SAM domain does not show any signal above background. Cells expressing TAp73α, which has a SAM domain, do not show staining either demonstrating the specificity of the DARPin A5 for the p63 SAM domain. A control DARPin does not show any signal above background. Scale bar, 50 µm.