A Prokaryotic genera for which the relative abundance of rRNAext was >1% in at least one sample. The panels from left to right indicate the relative abundances of these genera in the 16 S rRNAgenecell, rRNAcell, and rRNAext pools, respectively. B–D Principal Coordinate Analyses (PCoA) of the community structure of the prokaryotic ASVs for 16 S rRNAgenecell (B), rRNAcell (C), and rRNAext (D). The PCoA ordinates the weighted Unifrac metrics that are based on the presence/absence and the relative abundance of ASVs detected in each fraction. E Differences in the relative abundance of taxa at the genus level between May and June revealed by Linear discriminant analysis Effect Size (LEfSe). Panels from top to bottom showed the data for the 16 S rRNAgenecell, rRNAcell, and rRNAext fractions, respectively. The absolute value of the logarithmic LDA score indicates the degree of difference in the relative abundance of taxa between the two months.