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. 2022 Dec 14;12:21587. doi: 10.1038/s41598-022-26198-8

Table 2.

Primers and probes for qPCR quantification of stx2d55.

Gene Primer or probe Sequence (5′–3′) Position (5′–3′) Accession number
stx2 stx2-F TCA GGC AIA TAC AGA GAG AAT TTC G 578–602 AY443044
stx2-Ra CCG GIG TCA TCG TAT ACA CAG 646–626 AY443044
stx2-PΦ CAC TGT CTG AAA CTG CT 608–624 AY443044

Ф Probe tagged with minor groove-binding non-fluorescent quencher (MGBNFQ) and 6-carboxyfluorescin (FAM) fluorescent label (Eurogentec).