FIGURE 6.

PKHB1 peptide may enhance the local immune response by promoting the occurrence of ICD in infected cells. (A) HSK in vitro infection model in HCECs. (B) Infected HCECs were treated with PBS or PKHB1 peptide and the expression levels of HSV-1 gB were measured using qRT-PCR. GAPDH was used as an internal reference. PKHB1 peptide did not directly inhibit HSV-1 replication, but increased the release of more immunostimulatory factors from infected cells. (C) CALR exposure was observed by microscopy in HCECs using CALR staining and DAPI. Representative graphs of HMGB1 (D) or ATP (E) release in the extracellular supernatants of PKHB1 or PBS-treated cells (n = 3). Data were presented as mean ± SD of three independent experiments. (*p < 0.05, **p < 0.01, ***p < 0.001)