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. 2022 Dec 1;15:1007699. doi: 10.3389/fnmol.2022.1007699

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Copyright © 2022 Andersson, Fornasier, Makasewicz, Pálmadóttir, Linse, Sparr and Jönsson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Schematic illustration of the experimental procedure. Vesicles from two differently labeled populations are incubated together. After incubation, the vesicles are docked onto a passivated glass surface and imaged in two wavelength channels. The images are analyzed by detecting the positions, number of vesicles, and vesicle intensity. (B) Fusion between two differently labeled vesicles results in a reduced number of vesicles and colocalization of vesicles in the two wavelength channels with intensities similar to before vesicle mixing. (C) Lipid exchange results in an unchanged number of vesicles and colocalization between the vesicles in the two wavelength channels but with varying intensities compared to before vesicle mixing. (D) Fission, where only one vesicle population is needed, results in an increase in the number of vesicles and a decrease in the vesicle intensity.