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. 2022 Dec 1;15:1007699. doi: 10.3389/fnmol.2022.1007699

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Copyright © 2022 Andersson, Fornasier, Makasewicz, Pálmadóttir, Linse, Sparr and Jönsson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Fluorescence images of two samples of vesicles, either all vesicles are blue or red (0% colocalization) or all vesicles are both blue and red (100% colocalization). The red and blue channels are merged, and the scale bars are 10 μm. (B,C) The measured colocalization as a function of theoretical colocalization for data that is not drift corrected (green) and data that is drift corrected (black). The data corresponds to mean ± SD from n = 3–5 measurements. The solid lines are linear fits to the drift corrected data. (D,E) The integrated intensity I_tot (Eq. 4) distribution for the vesicle population collected from 1 to 3 measurements. Detected vesicles correspond to vesicles that are detected in the red (blue) channel and 0 and 100% lipid exchange refers to values when the mask from the blue (red) channel is applied to the red (blue) channel for samples with 0 and 100% lipid exchange.