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. 2022 Jul 13;29(12):2519–2530. doi: 10.1038/s41418-022-01037-5

Fig. 1. Flow cytometry analysis identifies B cell parameters affected by epigenetic modifying compounds.

Fig. 1

a Experimental workflow. CTV-labeled naïve murine B cells were cultured in LPS and treated with various compounds(1 µM). Half of the culture was harvested for flow cytometry analysis and transcriptome analysis was performed on 5000 cells per condition at 24 hr and 72 hr. b Total cell number and c mean division number at 72 hr post culture. d Representative CTV profiles for YKL-5-124, JQ1, A485, Vorinostat, GSK503 and ML324. Untreated controls are in gray. e Percentage of CD138+ cells at 72 hr. f Representative plots of CD138 expression for untreated, YKL-5-124, JQ1, A485, Vorinostat, GSK503 and ML324. g Summary on the effect of compounds on parameters measured by flow cytometry. Data in d, f are representative of two independent replicates. Significant differences in b, c, e were determined using ANOVA Bonferroni corrections. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.