Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Dec 14;13:7748. doi: 10.1038/s41467-022-35378-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a Proliferation kinetic of Huh-7 cells (ARID1A wild-type) transfected with control siRNA, ARID1A siRNA, TEAD1 siRNA or both. b Proliferation kinetic of Huh-7 cells transfected with control or ARID1A siRNA and treated with DMSO or verteporfin (1uM). c Dose-response curve of verteporfin in Huh-7 cells transfected with control or ARID1A siRNA. d, e Immunoblot of PARP (d) and apoptosis assay using AnnexinV and propidium iodide (PI) staining (e) of Huh-7 cells transfected with control or ARID1A siRNA 48 h after treatment with DMSO or verteporfin (1 uM). Immunoblot quantification is relative to loading control (actin). f Schematic representation of the CAM assay. g, h Representative pictures (g) and relative volume quantification (h) of tumors explanted from the CAM and derived from Huh-7 cells transfected with control siRNA or ARID1A siRNA and treated with DMSO or verteporfin (1uM). Error bars represent mean (+/−SD) from n ≥ 2 replicated. For all experiments performed, statistical significance was assessed by two-sided multiple t-tests (*P < 0.05, **P < 0.01, ***P < 0.001). f was generated using BioRender.