Fig. 4. Lysosomal acidic condition-induced assembly of DCNC.
a Representative confocal microscopy images of MCF-7 cells treated with different endocytosis inhibitors and then incubated with 1 μM DCNC for 6 h. Ami amiloride, Nys nystatin, Cpz chlorpromazine, Gen genistein. b Representative confocal microscopy images of MCF-7 cells after incubation with DCNC for varied time durations. Cell nuclei were stained with Hoechst 33342 (blue); late endosome/lysosomes were stained with LysoTracker (green). c Representative confocal microscopy images of MCF-7 cells that were incubated with DniCNC and DCNC for 48 h, respectively. d The line-scanning plots for fluorescence intensity quantitative analysis. e, f Representative TEM images and partial enlargement of MCF-7 cells that were treated with DniCNC (e) and DCNC (f), respectively, for 6 h. The white dotted line indicated lysosomes; the red arrows indicated nanocomplex (e) and aggregates (f).