Fig. 6. Preservation effects of DCNC towards cells.

a Schematic illustration of mitochondria damage caused by high-concentration H₂O₂. b Representative fluorescence microscopy images showing the JC-10-staining MCF-7 cells after incubation with DCNC for different time and then treated with H₂O₂. c Relative concentration of intracellular ATP. Bars represent mean ± SD (n = 3 independent samples). (*P < 0.05; ***P < 0.001, calculated by two-tailed unpaired t test). d Representative microscopy images of MCF-7 cells treated with a series of materials in a Transwell cell invasion device. e Cell viability of MCF-7 cells after incubation with different materials and treatment with H₂O₂. Bars represent mean ± SD (n = 5 independent samples). (***P < 0.001; ****P < 0.0001, calculated by two-tailed unpaired t test). f Representative fluorescence microscopy images showing living/dead cell assay of the MCF-7 cells treated with H₂O₂ and different materials. Living and dead MCF-7 cells were stained with calcein-acetoxymethylester (calcein-AM, green) and propidium iodide (PI, red), respectively.