TABLE 3.
Strains used in this work.
| Strain | Genotype | Phenotype/Use | Reference/Source |
| E. coli DH5α | supE4 ΔlacU169 (ϕ80 lacZΔM15) hsdR17, recA1, endA1, gyrA96, thi-1 relA1 | Cloning | |
| Thermus thermophilus strains | |||
| HB27 | ATCC BAA-163/DSM7039 | wild type | Koyama et al., 1986 |
| HB27pyrE | ΔpyrE:kat | KnR, Chromosome labeled | García-Quintans et al., 2020 |
| HB27ago | Δago | Hypercompetent | Blesa et al., 2015 |
| HB27ppol:lox72 | Δppol::lox72, Δ617578-631463, see Suplementary Table 3 | Hypercompetent | García-Quintans et al., 2020 |
| HB27ppol:kat | Δppol:kat, addA, see Supplementary Table 3 | KnR, Hypercompetent | García-Quintans et al., 2020 |
| HB27 ppol_cat | ppolcat, addA, see Supplementary Table 3 | Hypercompetent | This work |
| HB27 ppol_comp | Δ 617578-631463, see Supplementary Table 3 | Hypercompetent | This work |
| HB27 addAB:lox72 | ΔaddAB::lox72, ΔpTT27, see Suplementary Table 3 | Hypercompetent | This work |
| HB27 addAB:kat | ΔaddAB:kat, see Supplementary Table 3 | KnR, Hypercompetent | This work |
| HB27 addAB_ppol | ΔaddAB:lox72, Δppol:lox72, see Supplementary Tables 3, 4 | Hypercompetent | This work |