TABLE 4.
Plasmids used this work.
| Plasmid | Description/Use | References |
| pMotK1103A | Bifunctional modular vector, kat | Verdu et al., 2019 |
| pMotH1103A | Bifunctional modular vector, hyg | Verdu et al., 2019 |
| pUC19 | Cloning in Eco of construct for deletion mutants, AmpR | Vieira and Messing, 1982 |
| pyrEK | Suicide plasmid in T.th. AmpR (Eco), Deletion of pyrE gene with kat | García-Quintans et al., 2020 |
| pyrEH | Suicide plasmid in T.th. AmpR (Eco), Deletion of pyrE gene with hyg | García-Quintans et al., 2020 |
| pUC19:TTC0313:hyg | Suicide plasmid in T.th. AmpR (Eco), Deletion of TT_C0313 gene with hyg | Álvarez et al., 2014 |
| pD2lox | Suicide in T.th. Deletion of ppol by insertion of kat flanked by lox sites | García-Quintans et al., 2020 |
| pUC19:addAB:kat_lox | pUC19:addAB:kat. Suicide in T.th. Deletion of addAB by insertion of kat flanked by lox sites | This work |
| p174Cre | Bifunctional. Expression of Cre in T.th., hyg | García-Quintans et al., 2020 |
| pTTCC | Shuttle vector for CaldoCas9 expression in T. thermophilus | Adalsteinsson et al., 2021 |
| pTTCC_Ppol | pTTCC with a ppol-targeting guide cloned for ppol cat insertion in the genome | This work |
| pTTCC_Comp | pTTCC with guide for ppol insertion in ppol:lox72 strain | This work |
| pUC19_Template_CatPpol | Homologous recombination template for ppol cat CRISPR-insertion in wild type strain | This work |
| pUC19_Template_CompPpol | Homologous recombination template for ppol CRISPR-insertion in ppol:lox72 | This work |