FIGURE 3.

5-HT2A modulates protein body content. (A) 5-HT2A ^−/− mutants show reduced protein levels relative to controls, as measured by BCA (n = 10 where each replicate is comprised of 5 flies, one-sided t-test p = 0.019) (B) but excrete the same levels of protein as controls (n = 7 and 8 where each replicate is comprised of 20 flies, one-sided t-test p = 0.16). (C) 5-HT2A ^−/− mutants show no differences in triglyceride (TAG) levels relative to controls (n = 10 where each replicate is comprised of 5 flies, two-sided t-test p = 0.56). 5-HT2A ^−/− mutants show a significant reduction in (D) wet mass (n = 15–17 where each replicate is comprised of 8 flies, two-sided t-test p = 0.02) and (E) dry mass relative to controls (two-sided t-test p = 0.01). (F) Lifespan of control flies is shortened by mating (n = 153 and 146, log-rank analysis p = 0.002), while the and the lifespan of 5-HT2A ^−/− mutants is not (n = 151 and 134, log-rank analysis p = 0.3) and is not significantly different from control virgin lifespan (n = 151 and 146, log-rank analysis p = 0.9). All experiments were conducted in females.