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. 2022 Dec 15;8:491. doi: 10.1038/s41420-022-01280-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Spleen, kidney, lung and heart did not show gross pathological change after I3C i.g. administration at 2000 mg/kg. Only in the liver (red square), we observed congestion areas connected to the blood stasis zone. B Spleen, kidney, lung and heart showed no gross pathological change after I3C i.p. administration at 550 mg/kg. We only observed congestion areas in the liver (red square) connected to the blood stasis zone. Congestion was stained with H&E, Black arrows indicate congestion and containing red blood cells. C The number of congestions containing red blood cells in each section after I3C i.g. administration was counted. Results are presented as the average number of congestions per mm² as mean ± SD (n = 25–30 fields) from three biological replicates and two different observers. D The number of congestions containing red blood cells in each section after I3C i.p. administration was counted. Results are presented as the average number of congestions per mm² as mean ± SD (n = 25–30 fields) from three biological replicates and two different observers. Statistical significance was determined by one-way analysis of variance, ***p < 0.001.