TABLE 1.

PCR primers for detection of bovine cytokine transcripts in MAC-T cells

Primer	Sequence (5′ → 3′)	Predicted size (bp)	GenBank accession no.
5′ IL-1β	CACCTGGACCTCGGTTCCATGGGA	370	M37211
3′ IL-1β	CAAAGCTCATGCAGAACACCACTT
5′ IL-6	AATGAGAAAGGAGATATGTGAGAA	405	X57317, S49716
3′ IL-6	CTGAACTGCAGGAAATTCTCAAGG
5′ IL-8	ATGACTTCCAAACTGGCTGTTGCT	293	S82598
3′ IL-8	TTCTCAGCTCTCTTCACAAATACC
5′ TGF-β1	CTACTACGCCAAGGAGGTCACCC	319	M36271
3′ TGF-β1	CAGCCACTGCCGCACAACTCCAG
5′ TNF-α	ACTTCGGGGTAATCGGCCCCCAGA	345	AF011926
3′ TNF-α	CCTTGGTCTGGTAGGAGACTGCAA
5′ β-actin	TACCACCACAGCCGAGCGGGAAAT	455	K00622
3′ β-actin	GGAAGGTGGACAGGGAGGCCAGGA