Table 2.
Presence of antibiotic resistance and virulence genes in OMVs of NUHL30457 strain and 700,603 transformant.
| Gene | Results of PCRs performed with the following templates | |||||
|---|---|---|---|---|---|---|
| PK/DNase+ OMVs intacta | OMVs Lysed with TritonX-100 + b | Purified DNA Of Omvs | 700,603 transformant | Total DNA 30457 | Total DNA 700603 | |
| bla KPC | +c | -c | + | + | + | − |
| bla NDM | + | − | + | + | + | − |
| rmpA | − | − | − | − | + | − |
| rmpA2 | + | − | + | + | + | − |
| bla SHV | − | − | − | − | + | − |
| acc(6′)-Ib | − | − | − | − | + | − |
| bla CTX-M-9 group | + | − | + | + | + | − |
| iroB | + | − | + | + | + | − |
| iutA | + | − | + | + | + | − |
| silS | + | − | + | + | + | − |
a, OMVs treated by PK/DNase (to remove phage coats and extravesicular DNA) and left intact. b, OMVs lysed with Triton X-100. + indicates presence of amplicon; − indicates absence of amplicon.