Table 3.
Different treatments of vesicle-mediated transformation.
| Group | treatment | Amount (ug) | transformation | ||
|---|---|---|---|---|---|
| cfu/ml of transformants | Transformation frequency | Percentage of transformants | |||
| 1 | OMVs previously lysed with Triton X-100 | 20 | 0 | 0 | 0 |
| 2 | OMVs previously lysed with Triton X-100 | 50 | 0 | 0 | 0 |
| 3 | the free plasmid of 30,457 | 6 | 0 | 0 | 0 |
| 4 | OMVs treated with DNase I and proteinase K | 20 | [(2.8 ± 0.03)x108]a | [(1.4 ± 0.02)x10−4]a | [(1.4 ± 0.03)x10−2]a |
| 5 | OMVs treated with DNase I and proteinase K | 50 | [(3.79 ± 0.02)x108]a | [(7.58 ± 0.05)x10−4]a | [(7.58 ± 0.05)x10−2]a |
The data were expressed as mean ± standard deviation (SD) of three measurements; Means with lowercase letter “a” in the same column are significantly different at p < 0.05.