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. 2022 Dec 1;13:1047367. doi: 10.3389/fimmu.2022.1047367

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Copyright © 2022 Wu, Zhang, Zheng, Zhang, Cao, Sun and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Non-negative matrix factorization (NMF) analysis for the IRI samples. (A) Consensus map of NMF clustering when k = 2. (B) Distribution of cophenetic, residuals, RSS and silhouette with a rank of 2–10. (C) A PCA plot of the expression profile of DE-NRGs between two clusters. (D): Heatmap showing the expression landscape of DE-NRGs and the clinical characteristics of the two clusters. (E): Box plot showing the expression of 16 DE-NRGs between the two clusters. (F, G): Histogram comparing the differences in donor between the two clusters. (H, I) Histogram comparing the differences in the occurrence of DGF between the two clusters. IRI, ischemia reperfusion injury; RSS, residual sum of squares; PCA, principal component analysis; DE-NRGs, differentially expressed NET-related genes; DGF, delayed graft function. *P < 0.05, **P < 0.01, ***P < 0.001.