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. 2022 Dec 1;13:1047367. doi: 10.3389/fimmu.2022.1047367

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Copyright © 2022 Wu, Zhang, Zheng, Zhang, Cao, Sun and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Experimental verification processes. (A): The surgical process of renal ischemia-reperfusion in mouse. (B): H&E staining of the renal tissues from Sham mice, IRI mice and IRI+ G31P mice. (C, D): Serum creatinine and BUN were measured in the Sham group, the IRI group and the IRI+G31P group. (E): mRNA expression of Cxcl1 in kidney tissue of the control group and the IRI group. (F): Immunohistochemistry staining of NE in Sham mice, IRI mice and IRI+ G31P mice. (G–I): NET-related markers (dsDNA, MPO, NE) in the serum of the Sham group, the IRI group and the IRI+G31P group. H&E, hematoxylin and eosin; IRI, ischemia reperfusion injury; NE, neutrophil elastase; MPO, myeloperoxidase. **P < 0.01, ***P < 0.001.