FIG. 5.
Fractions of catalase C-10 at a Ve of 13.6 (peak C-10a) inhibit IFN-γ- and LPS-activated J774.16 macrophage production of RNI with concomitant enhancement of ornithine generation. Eluents were collected as 0.3-ml fractions. Fractions were added at a volume of 20 μl. Experiments were set up as described in the legend to Fig. 2. The phosphate buffer used did not attenuate RNI production or increase generation of ornithine. Data shown represent those of three independent experiments. Error bars indicate standard errors. Asterisks indicate a P value of <0.05 (one-way analysis of variance; controls were cultures without supplementation of C-10 or C-10a).