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. 2022 Sep 12;107(6):1540–1550. doi: 10.1093/biolre/ioac174

Figure 4.

Figure 4

ChIP-seq analysis on hypoxic hMSMCs (A–C) and verification of effects of HIF-1α on hMSMCs contractility (D–F). (A) Pie charts depicting the proportion of ChIP-seq peaks of HIF1A that cover each genomic region annotation. Annotations are derived from ChIPseeker. (B) The line plot and heatmap show the intensity of HIF1A ChIP-seq signals centered at the H3K27ac peak in hypoxic hMSMCs. (C) ChIP-seq tracks for HIF1A and H3K27ac at the genome regions of Gja1, Ptgs2 and Oxtr in hMSMCs. (D) Expressed mRNA levels of indicated genes by q-PCR (n = 4). (E) Expressed levels of indicated proteins by Western blot (n = 4). Control: cells under normoxia; hypoxia: cells under hypoxia for 2 h; siHIF1A: cells with HIF1A knocked down, treated with hypoxia for 2 h; NC: cells with siRNA negative control, treated with hypoxia for 2 h. *P < 0.05, **P < 0.01.