Fig. 3. (a) Pseudo-3D DOSY plot of the Oneshot–HSQC spectrum of mixture of five B vitamins (biotin, B7; pyridoxine hydrochloride, B6; pantothenic acid calcium complex, B5; niacin, B3; and thiamine hydrochloride, B1) in DMSO-d6. (b) 2D projection along F2, and (c) 2D projection along F1. F1 and F2 are the 13C and 1H dimensions, respectively. Dashed lines are used to aid visualization and indicate the peaks of biotin, B7 (red); pyridoxine hydrochloride, B6 (purple); pantothenic acid, B5 (blue); niacin, B3 (orange); and thiamine hydrochloride, B1 (green). The spectra from the five components are easily distinguished, with diffusion coefficients of (1.05, 1.12, 1.18, 1.36, and 1.64) × 10−10 m2 s−1 for vitamins B1, B7, B5, B6, and B3, respectively. Data acquisition took 45 h, but a much shorter acquisition would have sufficed. Reproduced with permission from ref. 51, © 2018 American Chemical Society.
