Fig. 7.

The NAT10 suppressor role of remodelin in CRC cells in vitro. A Effects of NAT10, KIF23, and β-catenin expression levels on CRC patient overall survival. B The IC50 value of remodelin was calculated in SW480 cells by CCK-8 assay. C RIP and acRIP followed by qPCR with the treatment of LiCl (20 mmol/L) or DMSO in SW480 cells for 48 h. D The expression of NAT10, KIF23, and β-catenin were detected by WB in SW480 cells with the treatment of LiCl (20 mmol/L) or DMSO for 48 h. E–G CCK-8, colony formation, and EdU assays were performed in SW480 cells with the treatment of LiCl (20 mmol/L) or DMSO for 48 h. H and I The flow cytometry of cell cycle and apoptosis were performed in SW480 cells with the treatment of LiCl (20 mmol/L) or DMSO for 48 h. J and K Transwell and wound healing assays were performed in SW480 cells with the treatment of LiCl (20 mmol/L) or DMSO for 48 h. Data are shown as mean ± SD of three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001