Fig. 2.

Quercetin induced apoptosis of HEC-1-A cells. a Apoptotic assay by DAPI staining. Cells were treated with quercetin for 24 h, and then the apoptotic cells were detected by DAPI staining and visualized under a fluorescent microscope. b Cell apoptosis detection by flow cytometry. Cells were treated with quercetin for 24 h, and the apoptotic cells were detected by staining with PI and Annexin V-FITC/PI followed by flow cytometry. Data are expressed as mean ± SD (n = 3). *p < 0.05, ** p < 0.01 vs. Control