FIG. 3.

Cytokine production by antigen-stimulated spleen cell cultures. BALB/c mice (five per group) were immunized as described in the legend to Fig. 1 and killed 10 days after challenge infection with MoPn. Spleen cells were incubated with 2 × 10⁵ IFU of UV-killed MoPn EBs in RPMI-1640 with 10% fetal calf serum and 5 × 10⁻⁵ M 2-mercaptoethanol in 5% CO₂ at 37°C. The supernatant was collected after 96 h of incubation. IFN-γ (A) and IL-10 (B) in the supernatant were tested by ELISA. The data are means ± SEM. ∗∗, P < 0.01 compared with N, IS, and pc/IS.