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. 2022 Nov 7;41(24):e111648. doi: 10.15252/embj.2022111648

Figure EV1. Pup retrieval behavior in virgin female mice.

Figure EV1

  1. Total pup retrieval time of virgin females in the follicular or secretory stage of the estrus cycle (n = 4/group). Data were analyzed using two‐way ANOVA with repeated measures and are expressed as mean ± s.e.m.
  2. Representative images of vaginal smears of pre‐estrus, estrus, metestrus, and diestrus stage.
  3. Schematic representation of the pup retrieval test at different time points.
  4. Total pup retrieval time in the first and second rounds of behavioral testing in virgin female mice (n = 6/group). Data were analyzed using two‐way ANOVA with repeated measures and are expressed as mean ± s.e.m. ****significantly different from foster mothers, P < 0.0001; ####significantly different from mothers, P < 0.0001.
  5. Total pup retrieval time in virgin females exposed to pups at different ages (P0 and P2) on the first day of pup exposure (P0: n = 10: P2: n = 7). Data were analyzed using Student's t‐test and displayed as individual values ± s.e.m.

Source data are available online for this figure.