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A, B
TNF increases maximal respiration (A) and spare respiratory capacity (B). HeLa cells were treated with TNF for the indicated time and analyzed by the Agilent Seahorse XF Cell Mito Stress Test. The spare respiratory capacity is the difference between maximal and basal respiration. One‐way ANOVA with Tukey's multiple comparisons test, n = 4 independent experiments, bars represent mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.
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C
TNF increases total ATP production. HeLa cells were treated with TNF for indicated time and analyzed by the Agilent Seahorse ATP Rate Assay. One‐way ANOVA with Tukey's multiple comparisons test, n = 3 independent experiments, bars represent mean ± SEM. ***P < 0.001.
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D
PINK1 silencing decreases NF‐κB transcriptional activity. Hela cells were treated with PINK1‐specific or control siRNA. The next day, cells were transfected with an NF‐κB luciferase reporter construct. Twenty‐four hours after transfection, cells were treated with TNF (25 ng/ml, 3 h) harvesting. Luciferase activity was quantified in cell lysates with luciferase activity in untreated control cells set to 1. Data represent the mean with standard deviation. Sidak's multiple comparisons test, n = 3 independent experiments, *P < 0.05, ***P < 0.001.
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E
Miro1/2 silencing decreases NF‐κB transcriptional activity. Hela cells were treated with Miro 1/2‐specific or control siRNA. The next day, cells were transfected with an NF‐κB luciferase reporter construct and analyzed as described in (A). Data represent the mean with standard deviation. Sidak's multiple comparisons test, n = 3 independent experiments, n.s., not significant, *P < 0.05.
