FIG. 1.

Mapping of the dominant T-cell determinants of the MalE protein in C57BL/6 mice. C57BL/6 mice were primed s.c. with 10 μg of MalE protein emulsified in CFA. Ten days later, LN cells were stimulated in vitro with overlapping 15-mer peptides (6.5 μM concentration) covering the entire MalE sequence in a single amino acid step, which is referred as the NH₂-terminal amino acid of each peptide. Proliferation was determined by ³H incorporation on day 4.