FIG. 3.

Analysis of the MalE-specific T-cell repertoire stimulated in vivo by SL3261*pMalE. C57BL/6 mice (two per group) were i.p. immunized on days 0 and 21 with 10⁶ SL3261*pMalE or control SL3261* bacteria. Spleen cells from each group were pooled and were stimulated in vitro with the indicated Ag. (A) Indicated MalE synthetic peptides were tested individually at 10 μM, purified MalE was tested at 0.25 μM, and SL3261* extract was tested at a dilution corresponding to 10⁶ bacteria/ml. (B) Series of 12 peptides were pooled (2 μM concentration of each peptide) and tested for reactivity. The peptide pools are referred to as the sequence encompassed by peptide series. Proliferation was determined by ³H incorporation on day 4. Asterisks indicate positive proliferative responses corresponding to those depicted in Fig. 1.