FIG. 4.
Stimulation of p40–54-specific T-cell hybridoma by purified MalE or SL3261*pMalE. (A) The 53C1 T-cell hybridoma was stimulated by a 10-μM concentration of synthetic peptides corresponding to residues 35 to 60 from MalE in the presence of an I-Ab transfected L cell. (B) PEC were incubated for 2 h with SL3261*pMalE or SL3261*. After fixation these cells were used to stimulate the 53C1 T-cell hybridoma. (C) PEC were incubated with 10 μg of MalE per ml or with 106 SL3261*pMalE bacteria per ml alone, with 10 μM BFA, or with 10 μM CCB. The IL-2 content in the 24-h supernatant was measured using the CTLL cell line.