FIG. 5.

The crypticity of p40–54 following immunization with purified MalE in adjuvant is not due to inefficient processing in vivo. (A) Mice were s.c. immunized with 10 μg of MalE or p40–54 in CFA. Ten days later, LN cells were restimulated with serial dilutions of the p40–54 peptide. Proliferation was determined by ³H incorporation on day 4. (B, C, and D) Mice were s.c. immunized with MalE in adjuvant. Three days later, T-cell-depleted LN cells were directly used as APC to stimulate T-cell hybridomas specific for p40–54 (53C1) or p221–235 (ORMC7.9). (B) One hundred micrograms of MalE was administered in IFA or alum as indicated. (C and D) Indicated doses of MalE were injected together with IFA.