FIG. 6.

Salmonella delivery of MalE does not modify the MHC presentation pattern of epitopes by Mφ and DC. PEC (A, B, and C) and DC (D, E, and F) were incubated for 2 and 3 h, respectively, with serial doses of SL3261*pMalE or purified MalE. After fixation these cells were used to stimulate the following T-cell hybridomas: ORM.C7.9 specific for p221–235 (A and D), 53C1 specific for p40–54 (B and E), or TRM.F2.1 specific for p262–276 (C and F). The IL-2 content in the 24-h supernatant was measured using the CTLL cell line.