FIG. 4.
The expression of CD44 and CD45RB on CD4+ T cells from the spleens of uninfected, M. avium-infected, or DNA-35-immunized, and M. avium-infected mice were analyzed by flow cytometry. The profiles are representative of five mice from one of two experiments. Numbers in quadrants are percentages of CD4+ T cells (A). The number of CD44hi CD45RBlo CD4+ T cells in the spleens from mice in the seven groups shown were analyzed at 2, 4, 8, and 14 weeks. The data are the mean ± SEM from five mice per group from one of two experiments. The differences between DNA-35- and BCG-immunized groups were determined by the Mann-Whitney test where P < 0.01 at weeks 4 and 8, as indicated.