FIG 2.

Maintenance of pKW-L2ori and pKW-CMori by chlamydiae. (A) Fluorescent images of live C. trachomatis serovar L2 inclusions transformed with pKW-L2ori in McCoy cells at 30 h postinfection. (B) qPCR analysis of pKW-L2ori vector maintenance and elimination in four consecutive passages of L2 in the presence or absence of spectinomycin. (C) Fluorescent images of live C. trachomatis serovar D inclusions transformed with pKW-L2ori in McCoy cells at 40 h postinfection. (D) qPCR analysis of the pKW-L2ori vector in four consecutive passages of serovar D, incubated with or without spectinomycin. (E) Fluorescent images of live C. muridarum inclusions transformed with pKW-CMori in McCoy cells at 24 h postinfection. (F) qPCR analysis of pKW-CMori vector maintenance and elimination in four consecutive passages of C. muridarum in the presence or absence of penicillin G. The gfp gene copy number was relative to the 16S rRNA region. Scale bar = 10 μM.