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Maps of the pJB06 (cas9-containing) and pJB07 (homology- and gRNA-encoding) plasmids. The pJB06 and pJB07 plasmids have been engineered to carry both the Tn916 oriT and traJ regions for conjugal transfer from B. subtilis or E. coli, respectively. Both the cas9 gene and the gRNA are under the conditional expression of a xylose-inducible promoter. The pJB06 plasmid is maintained in a strain of interest, and modified pJB07 plasmids are introduced to generate the desired mutations.
