TABLE 1.

Primers and gBlocks used in the construction of pJB06 and pJB07

Primer name	Primer sequence
5′PxylR_COcas9	TTATCAGGAAACAGCTATGACCGCGGCCGCCCCTTATATTTCATTAATTAAAGTTAAATT
3′PxylR_COcas9	TGCCAAGCTTGCATGTCTGCAGGCCTCGAGTTAATCACCACCTAATTGAGATAAATC
5′Tn916_ori_Gibson	CGGAAGAGCGCCCAATACGCAGGGCCCTAACATCTTCTATTTTTCCCAAATC
3′traJ	AATTTATCTACAATTTTTTTATCCTGCAGGGGGCCCGATCGGTCTTGCCTTG
5′pyrE_UP	TTATCAGGAAACAGCTATGACCGCGGCCGCGACGTGATTTTTAATGGGTA
3′pyrE_DN	TGCCAAGCTTGCATGTCTGCAGGCCTCGAGAAGCATTGATGTTCTTCCTTC
pyrE_gRNA_PxylR	GCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCTAGCTGACAAAGATAATTAAATATTTTATTATTAGTTCATAAGTTAGTTTAATATACTAACAAAAATAAAGCAAGTAAAATATACCTAAAATATAAAAAAATTAGGATAGGAAAACGATAGTTATGAAGTGGCATTCAAGGAGGGGGTACCGAAAAGTGATGCATTGTTGGGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTTCTATGGAGAAATCTAGATCAGCATGATGTCTGACTAGACGCGTGCTAGCATAAAAATAAGAAGCCTGCATTT