TABLE 1.
Oligonucleotide and antibody resources used in this study
| Resource | ID or catalog no. | Additional information (sequence or concn used) |
|---|---|---|
| sgRNA for Ube3d KO | mUBE3D Exon2_sgRNA | CGAGATCCGACTTCCAGCAGGGG |
| mUBE3D Exon5_sgRNA | TAAGCGTTGCAAGGTAACGTTGG | |
| Primers for genotype sequencing | mUBE3D-Exon 2-F | GCAAGCCCTGCCTGATCTAA |
| mUBE3D-Exon 5-F | TCAGCAATGCTAGGATTGCCA | |
| 18S qPCR primers | 18S_F | ACCGCAGCTAGGAATAATGGA |
| 18S_R | GCCTCAGTTCCGAAAACCA | |
| Ube3d qPCR primers | mUBE3D_F | GCAGAGTGCGCTGCTAATC |
| mUBE3D_R | CTGGAAGTCGGATCTCGGT | |
| PparγqPCR primers | PPARg_F | CTCCAAGAATACCAAAGTGCGA |
| PPARg_R | GCCTGATGCTTTATCCCCACA | |
| Cebpa primers | C/EBPa_F | TTGTTTGGCTTTATCTCGGC |
| C/EBPa_R | CCAAGAAGTCGGTGGACAAG | |
| Fabp4 primers | FABP4_F | AAGGTGAAGAGCATCATAACCCT |
| FABP4_R | TCACGCCTTTCATAACACATTCC | |
| Cfd (adipsin) primers | Adipsin_F | CATGCTCGGCCCTACATGG |
| Adipsin_R | CACAGAGTCGTCATCCGTCAC | |
| Adipoq (adiponectin) primers | Adiponectin_F | TGTTCCTCTTAATCCTGCCCA |
| Adiponectin_R | CCAACCTGCACAAGTTCCCTT | |
| Alpha-tubulin monoclonal antibody (YOL1/34) | MA1-80189 (PaGenBase) | 1:3,000 |
| Anti-A-FABP antibody (B-4) | sc-271529 (Santa Cruz) | 1:150 |
| Anti-C/EBP α antibody (D-5) | sc-365318 (Santa Cruz) | 1:100 |
| Anti-C/EBP δ antibody (D-1) | sc-515028 (Santa Cruz) | 1:100 |
| Anti-CPSF3 antibody (C-3) | sc-393001 (Santa Cruz) | 1:1,000 |
| Anti-fibulin-3 antibody (mab3-5) | sc-33722 (Santa Cruz) | 1:100 |
| Anti-HGFα antibody (H-10) | sc-374422 (Santa Cruz) | 1:100 |
| Anti-LPL antibody (F-1) | sc-373759 (Santa Cruz) | 1:100 |
| Anti-OAZ (E-6) mouse MAba | sc-393904 (Santa Cruz) | 1:150 |
| Anti-PPARγ antibody (E-8) | sc-7273 (Santa Cruz) | 1:500 |
| Anti-SLPI antibody (A-11) | sc-374575 (Santa Cruz) | 1:100 |
| GPRIN3-specific polyclonal antibody | 20212-1-AP (Proteintech) | 1:500 |
| Human/mouse EBF-2 antibody | AF7006 (R&D Systems) | 1:500 |
| UBE3D polyclonal antibody | PAB21883 (Abnova) | 1:250 |
a
MAb, monoclonal antibody.