TABLE 3.
List of primers
| Primer name | Sequencesa | Position of the amplicon relative to estimated VINK TSS (nt) |
|---|---|---|
| VINK-A | FW: CTGGACACACCCTAGGCAAG RV: ATCCCTGTTGGTTATTCACAGC |
+207 to +323 |
| VINK-B | FW: CCACAACCCCCATCTCTCTG RV: CCCATCTTTGGTTCTTCTGTC |
+21,840 to +21,390 |
| VINK-C | FW: TGGGTAGCATCTTCATCGGTG RV: GAAGTGAGGGGAGTAGACAAG |
+22,497 to +22,751 |
| VINK-D | FW: ACGCATGGGTGCCCTGTTTG RV: ATAGATCTTGGAGGGTGCCAG |
+162,326 to +162,445 |
| VINK-E | FW: ACCACTATACAATTGCAGCAGG RV: CTGTCTGTTTAGGTACCTGGC |
+199,375 to +199,539 |
| VINK-F | FW: GAGTGTCATCAGTAGTCTTAGCC RV: CGAGGTCCAATATCCCTAACC |
+296,406 to +296,540 |
| 420 bp encompassing the deletion product PCR primers | FW: GCATGAGTCATCTGGCTGTG RV: CCCATCTTTGGTTCTTCTGTC |
|
| WT VINK allele primers | FW: CCACAACCCCCATCTCTCTG RV: CCCATCTTTGGTTCTTCTGTC |
|
| Δ VINK allele primers | FW: ATATAGGAGGCCAGTGCTCCCCATG RV: CCCATCTTTGGTTCTTCTGTC |
|
| GAPDH exon 9 primers | FW: TGACAACGAATTTGGCTACAGC RV: CTCTTCCTCTTGTGCTCTTGC |
|
| GAPDH promoter primers | FW: AAATTGAGCCCGCAGCCTCC RV: GCGACGCAAAAGAAGATGCG |
|
| ARHGAP18-EX1 | FW: CAGGCGAGACAGGAACTTCTTT RV: CTGCCTTTGCATGGCTGTTC |
|
| ARHGAP18-INT1 | FW: GCTGCTGGAGTGAAATGTGG RV: TGTTCAGCTAGTGAGAAGGTC |
|
| ARHGAP18-read_through | FW: GTTCCTAGAGATTGATCTGAGG RV: GAATAGACTTGGGTTGCCACG |
|
| AGO1 | FW: CAGTGGACACCAACATCACC RV: AAACGGTTGTCATCCCAAAG |
|
| AGO2 | FW: CGCGTCCGAAGGCTGCTCTA RV: TGGCTGTGCCTTGTAAAACGCT |
a
FW, forward; RV, reverse.