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Effect of bacterial incubation time on PS expression. HEp-2 cells were incubated with EHEC (85-170) for 2 h (dotted), 3 h (black), or overnight (grey). PS expression was determined by flow cytometric analysis of annexin V-FITC binding as for Fig. 1. Similar results (not shown) were achieved with EHEC strains 84-289 and CL56.
