FIG. 6.
Bacterial binding versus NBD-PE uptake. Flow cytometric dot plots show the fluorescence intensity of NBD-PE staining on the abscissa and bacterial binding (FITC staining) on the ordinate. Bacterial binding was determined for CL56 with cells treated with NBD-PE liposomes (A), cells pretreated with NEM and then NBD-PE liposomes (B), untreated cells (C), and cells pretreated with NEM (D). Additional controls (not shown) included cells incubated with primary and/or secondary antibody and cells incubated only with NBD-PE liposomes.